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human matrix metalloproteinase 9 mmp9  (R&D Systems)


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    Structured Review

    R&D Systems human matrix metalloproteinase 9 mmp9
    Human Matrix Metalloproteinase 9 Mmp9, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mmp+9/Recombinant+Human+MMP-9+Western+Blot+Standard+Protein/pm41814444-91-2-47
    Average 93 stars, based on 46 article reviews
    human matrix metalloproteinase 9 mmp9 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Modeling Vascular Inflammation and Atherogenicity after Inhalation of Ambient Levels of Ozone: Exploratory Lessons from Transcriptomics
    Article Snippet: .. The gelatinase activity of the proteins of two of the DEGs, MMP-9 and MMP-2, was measured in archived BAL and serum samples from subjects in the current ozone study using Novex Zymogram gels with 0.1% gelatin as a substrate (ThermoFisher Scientific, Inc., MA, USA) according to manufacturer instructions along with 1 ng of recombinant MMP-9 or MMP-2 control (R&D System, MN, USA), followed by staining with Coomassie Blue. .. The signals for pro- and active MMP-9 and MMP-2 were visualized using FluorChem FC2 System (Cell Biosciences, Santa Clara, CA, USA), and the optical density was measured using ImageJ software (version 1.44, NIH, MD, USA) by two separate blinded observers, averaged, adjusted for control, and then analyzed by regression modeling using STATA 12.0 software (STATA Corp, TX, USA).

    Recombinant:

    Article Title: Modeling Vascular Inflammation and Atherogenicity after Inhalation of Ambient Levels of Ozone: Exploratory Lessons from Transcriptomics
    Article Snippet: .. The gelatinase activity of the proteins of two of the DEGs, MMP-9 and MMP-2, was measured in archived BAL and serum samples from subjects in the current ozone study using Novex Zymogram gels with 0.1% gelatin as a substrate (ThermoFisher Scientific, Inc., MA, USA) according to manufacturer instructions along with 1 ng of recombinant MMP-9 or MMP-2 control (R&D System, MN, USA), followed by staining with Coomassie Blue. .. The signals for pro- and active MMP-9 and MMP-2 were visualized using FluorChem FC2 System (Cell Biosciences, Santa Clara, CA, USA), and the optical density was measured using ImageJ software (version 1.44, NIH, MD, USA) by two separate blinded observers, averaged, adjusted for control, and then analyzed by regression modeling using STATA 12.0 software (STATA Corp, TX, USA).

    Article Title: Soluble CD147 regulates endostatin via its effects on the activities of MMP-9 and secreted proteasome 20S
    Article Snippet: .. Supernatants derived from the experimental samples were then added to the ECM together with increasing concentrations of either MMP-9 inhibitor I, the proteasome 20S inhibitor AM-114, or recombinant MMP-9 (R&D Systems) that was activated with APMA (1 mM, for 24 h) or with a proteasome 20S preparation. ..

    Article Title: Chronic E-Cigarette Use Increases Neutrophil Elastase and Matrix Metalloprotease Levels in the Lung
    Article Snippet: Densitometric analysis of digitized Western blots was performed using NIH ImageJ software. .. To confirm the corresponding protein band of the proteases, we performed additional Western blots using purified human neutrophil elastase from sputum (Elastin Products Company, Inc #SE563), recombinant MMP-2 (Abcam #ab174022) and recombinant MMP-9 (R&D Systems #911-MP) and identified the representative bands of the relevant protease following antibody manufacturer’s instruction (Figure E7). ..

    Article Title: Soluble CD147 regulates endostatin via its effects on the activities of MMP-9 and secreted proteasome 20S
    Article Snippet: Some experiments included the addition of the rabbit anti-human CD147 antibody (h161-pAb, 2 ng/ml, produced in our lab), the human recombinant CD147 protein (R&D Systems, Minneapolis, MN, USA), or the human recombinant TIMP-1 (R&D Systems). .. In other experiments, the human recombinant MMP-9 (R&D Systems) and the human erythrocyte-derived 20S proteasome protein (R&D Systems) were added to the culture. ..

    Article Title: Neuropeptide Y Induces Hematopoietic Stem/Progenitor Cell Mobilization by Regulating Matrix Metalloproteinase-9 Activity Through Y1 Receptor in Osteoblasts.
    Article Snippet: For in vivo experiment, mice were injected with NPY, Y1 agonist, Y1 antagonist (50 lg per kg body weight, i.v.), MMP-9 neutralizing monoclonal antibody (Millipore, IM09L. .. 3mg per kg body weight, i.v.) or recombinant MMP-9 (R&D system, 909-MM-010. ..

    Article Title: Matrix Metalloproteinase-9 Mediates RSV Infection in Vitro and in Vivo
    Article Snippet: Immunoblots were then developed using ECL chemiluminescent kits (GE Healthcare, Piscataway, NJ, USA). .. 50 ng of recombinant MMP-9 (R/D Systems; Catalog #WBC018) was used as positive control. ..

    Article Title: Contribution of ADAMTS13-independent VWF regulation in sickle cell disease.
    Article Snippet: © 2022 International Society on Thrombosis and Haemostasis.. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.. Manuscript handled by: Karen Vanhoorelbeke Final decision: Karen Vanhoorelbeke, 23 June 2022 1Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA 2Confocal Imaging Facility, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA 3Division of Cardiac Anesthesia, Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA

    Control:

    Article Title: Modeling Vascular Inflammation and Atherogenicity after Inhalation of Ambient Levels of Ozone: Exploratory Lessons from Transcriptomics
    Article Snippet: .. The gelatinase activity of the proteins of two of the DEGs, MMP-9 and MMP-2, was measured in archived BAL and serum samples from subjects in the current ozone study using Novex Zymogram gels with 0.1% gelatin as a substrate (ThermoFisher Scientific, Inc., MA, USA) according to manufacturer instructions along with 1 ng of recombinant MMP-9 or MMP-2 control (R&D System, MN, USA), followed by staining with Coomassie Blue. .. The signals for pro- and active MMP-9 and MMP-2 were visualized using FluorChem FC2 System (Cell Biosciences, Santa Clara, CA, USA), and the optical density was measured using ImageJ software (version 1.44, NIH, MD, USA) by two separate blinded observers, averaged, adjusted for control, and then analyzed by regression modeling using STATA 12.0 software (STATA Corp, TX, USA).

    Staining:

    Article Title: Modeling Vascular Inflammation and Atherogenicity after Inhalation of Ambient Levels of Ozone: Exploratory Lessons from Transcriptomics
    Article Snippet: .. The gelatinase activity of the proteins of two of the DEGs, MMP-9 and MMP-2, was measured in archived BAL and serum samples from subjects in the current ozone study using Novex Zymogram gels with 0.1% gelatin as a substrate (ThermoFisher Scientific, Inc., MA, USA) according to manufacturer instructions along with 1 ng of recombinant MMP-9 or MMP-2 control (R&D System, MN, USA), followed by staining with Coomassie Blue. .. The signals for pro- and active MMP-9 and MMP-2 were visualized using FluorChem FC2 System (Cell Biosciences, Santa Clara, CA, USA), and the optical density was measured using ImageJ software (version 1.44, NIH, MD, USA) by two separate blinded observers, averaged, adjusted for control, and then analyzed by regression modeling using STATA 12.0 software (STATA Corp, TX, USA).

    Derivative Assay:

    Article Title: Soluble CD147 regulates endostatin via its effects on the activities of MMP-9 and secreted proteasome 20S
    Article Snippet: .. Supernatants derived from the experimental samples were then added to the ECM together with increasing concentrations of either MMP-9 inhibitor I, the proteasome 20S inhibitor AM-114, or recombinant MMP-9 (R&D Systems) that was activated with APMA (1 mM, for 24 h) or with a proteasome 20S preparation. ..

    Western Blot:

    Article Title: Chronic E-Cigarette Use Increases Neutrophil Elastase and Matrix Metalloprotease Levels in the Lung
    Article Snippet: Densitometric analysis of digitized Western blots was performed using NIH ImageJ software. .. To confirm the corresponding protein band of the proteases, we performed additional Western blots using purified human neutrophil elastase from sputum (Elastin Products Company, Inc #SE563), recombinant MMP-2 (Abcam #ab174022) and recombinant MMP-9 (R&D Systems #911-MP) and identified the representative bands of the relevant protease following antibody manufacturer’s instruction (Figure E7). ..

    Purification:

    Article Title: Chronic E-Cigarette Use Increases Neutrophil Elastase and Matrix Metalloprotease Levels in the Lung
    Article Snippet: Densitometric analysis of digitized Western blots was performed using NIH ImageJ software. .. To confirm the corresponding protein band of the proteases, we performed additional Western blots using purified human neutrophil elastase from sputum (Elastin Products Company, Inc #SE563), recombinant MMP-2 (Abcam #ab174022) and recombinant MMP-9 (R&D Systems #911-MP) and identified the representative bands of the relevant protease following antibody manufacturer’s instruction (Figure E7). ..

    Positive Control:

    Article Title: Matrix Metalloproteinase-9 Mediates RSV Infection in Vitro and in Vivo
    Article Snippet: Immunoblots were then developed using ECL chemiluminescent kits (GE Healthcare, Piscataway, NJ, USA). .. 50 ng of recombinant MMP-9 (R/D Systems; Catalog #WBC018) was used as positive control. ..

    Clinical Proteomics:

    Article Title: Contribution of ADAMTS13-independent VWF regulation in sickle cell disease.
    Article Snippet: © 2022 International Society on Thrombosis and Haemostasis.. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.. Manuscript handled by: Karen Vanhoorelbeke Final decision: Karen Vanhoorelbeke, 23 June 2022 1Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA 2Confocal Imaging Facility, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA 3Division of Cardiac Anesthesia, Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA

    Incubation:

    Article Title: Contribution of ADAMTS13-independent VWF regulation in sickle cell disease.
    Article Snippet: © 2022 International Society on Thrombosis and Haemostasis.. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.. Manuscript handled by: Karen Vanhoorelbeke Final decision: Karen Vanhoorelbeke, 23 June 2022 1Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA 2Confocal Imaging Facility, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA 3Division of Cardiac Anesthesia, Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA

    Produced:

    Article Title: Contribution of ADAMTS13-independent VWF regulation in sickle cell disease.
    Article Snippet: © 2022 International Society on Thrombosis and Haemostasis.. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.. Manuscript handled by: Karen Vanhoorelbeke Final decision: Karen Vanhoorelbeke, 23 June 2022 1Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA 2Confocal Imaging Facility, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA 3Division of Cardiac Anesthesia, Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA

    Transduction:

    Article Title: Contribution of ADAMTS13-independent VWF regulation in sickle cell disease.
    Article Snippet: © 2022 International Society on Thrombosis and Haemostasis.. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.. Manuscript handled by: Karen Vanhoorelbeke Final decision: Karen Vanhoorelbeke, 23 June 2022 1Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA 2Confocal Imaging Facility, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA 3Division of Cardiac Anesthesia, Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA

    Expressing:

    Article Title: Contribution of ADAMTS13-independent VWF regulation in sickle cell disease.
    Article Snippet: © 2022 International Society on Thrombosis and Haemostasis.. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.. Manuscript handled by: Karen Vanhoorelbeke Final decision: Karen Vanhoorelbeke, 23 June 2022 1Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA 2Confocal Imaging Facility, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA 3Division of Cardiac Anesthesia, Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA

    Construct:

    Article Title: Contribution of ADAMTS13-independent VWF regulation in sickle cell disease.
    Article Snippet: © 2022 International Society on Thrombosis and Haemostasis.. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.. Manuscript handled by: Karen Vanhoorelbeke Final decision: Karen Vanhoorelbeke, 23 June 2022 1Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA 2Confocal Imaging Facility, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA 3Division of Cardiac Anesthesia, Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA



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